Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 52, Pages E8379-E8386Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1608461113
Keywords
circulating tumor cells; microfluidics; rare-cell sorting; reverse transcription-PCR; single-cell analysis
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Funding
- NIH [U54CA151459, R21CA185804]
- Canary Foundation
- LUNGevity Foundation
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Circulating tumor cells (CTCs) are established cancer biomarkers for the liquid biopsy of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non-small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a high-throughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.
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