Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 40, Pages 11152-11157Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1606372113
Keywords
cytoskeleton; intermediate filaments; vimentin; self-assembly; time-resolved light scattering
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Funding
- German Research Foundation (DFG) [SFB 755, B07]
- DFG [KO 3572/5-1]
- Helmholtz Gemeinschaft in the framework of the Virtual Institute [VH-VI-403]
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Vimentin intermediate filaments (IFs) are part of a family of proteins that constitute one of the three filament systems in the cytoskeleton, a major contributor to cell mechanics. One property that distinguishes IFs from the other cytoskeletal filament types, actin filaments and microtubules, is their highly hierarchical assembly pathway, where a lateral association step is followed by elongation. Here we present an innovative technique to follow the elongation reaction in solution and in situ by time-resolved static and dynamic light scattering, thereby precisely capturing the relevant time and length scales of seconds to minutes and 60-600 nm, respectively. We apply a quantitative model to our data and succeed in consistently describing the entire set of data, including particle mass, radius of gyration, and hydrodynamic radius during longitudinal association.
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