Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 39, Pages 11004-11009Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1602751113
Keywords
E4F1; PDH; pyruvate; skin; stem cell
Categories
Funding
- Agence Nationale pour la Recherche (ANR)
- INSERM Avenir Program
- Institut National du Cancer
- Site de Recherche Integree sur le Cancer [INCa-DGOS-INSERM 6045]
- Association pour la Lutte Contre le Cancer (ARC)
- European Regional Development Fund (ERDF)-Languedoc Roussillon grant (Transportome)
- Laboratory of Excellence from Genome and Epigenome to Molecular Medecine (Labex EpiGenMed, a program of the French National Research Agency) [ANR-10-LABX-12-01]
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The multifunctional protein E4 transcription factor 1 (E4F1) is an essential regulator of epidermal stem cell (ESC) maintenance. Here, we found that E4F1 transcriptionally regulates a metabolic program involved in pyruvate metabolism that is required to maintain skin homeostasis. E4F1 deficiency in basal keratinocytes resulted in deregulated expression of dihydrolipoamide acetyltransferase (Dlat), a gene encoding the E2 subunit of the mitochondrial pyruvate dehydrogenase (PDH) complex. Accordingly, E4f1 knock-out (KO) keratinocytes exhibited impaired PDH activity and a redirection of the glycolytic flux toward lactate production. The metabolic reprogramming of E4f1 KO keratinocytes associated with remodeling of their microenvironment and alterations of the basement membrane, led to ESC mislocalization and exhaustion of the ESC pool. ShRNA-mediated depletion of Dlat in primary keratinocytes recapitulated defects observed upon E4f1 inactivation, including increased lactate secretion, enhanced activity of extracellular matrix remodeling enzymes, and impaired clonogenic potential. Altogether, our data reveal a central role for Dlat in the metabolic program regulated by E4F1 in basal keratinocytes and illustrate the importance of PDH activity in skin homeostasis.
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