Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 51, Pages E8286-E8295Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1612024114
Keywords
NKT cells; miRNA; TGF-beta; development; CD1d
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Funding
- Fondazione Cariplo [2009-3603]
- Italian Association for Cancer Research Grant [AIRC IG15466, IG15517]
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Invariant natural killer T cells (iNKT) cells are T lymphocytes displaying innate effector functions, acquired through a distinct thymic developmental program regulated by microRNAs (miRNAs). Deleting miRNAs by Dicer ablation (Dicer KO) in thymocytes selectively impairs iNKT cell survival and functional differentiation. To unravel this miRNA-dependent program, we systemically identified transcripts that were differentially expressed between WT and Dicer KO iNKT cells at different differentiation stages and predicted to be targeted by the iNKT cell-specific miRNAs. TGF-beta receptor II (TGF-beta RII), critically implicated in iNKT cell differentiation, was found up-regulated in iNKT Dicer KO cells together with enhanced TGF-beta signaling. miRNA members of the miR-17 similar to 92 family clusters were predicted to target Tgfbr2 mRNA upon iNKT cell development. iNKT cells lacking all three miR-17 similar to 92 family clusters (miR-17 similar to 92, miR-106a similar to 363, miR-106b similar to 25) phenocopied both increased TGF-beta RII expression and signaling, and defective effector differentiation, displayed by iNKT Dicer KO cells. Consistently, genetic ablation of TGF-beta signaling in the absence of miRNAs rescued iNKT cell differentiation. These results elucidate the global impact of miRNAs on the iNKT cell developmental program and uncover the targeting of a lineage-specific cytokine signaling by miRNAs as a mechanism regulating innate-like T-cell development and effector differentiation.
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