Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 113, Issue 5, Pages 1214-1219Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1524607113
Keywords
chromatin; nucleosome; DNA; X-ray structure; MMTV
Categories
Funding
- Swiss National Fund Grant [310030B_138742]
- European Research Council Grant [322778]
- Swiss National Science Foundation (SNF) [310030B_138742] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [322778] Funding Source: European Research Council (ERC)
Ask authors/readers for more resources
The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for two similar a-satellite sequences and a sequence (601) selected in vitro have been visualized bound in the nucleosome core. Here we report the 2.6-angstrom resolution -ray structure of a nucleosome core particle containing the DNA sequence of nucleosome A of the 3'-LTR of the mouse mammary tumor virus (147 bp MMTV-A). To our knowledge, this is the first nucleosome core particle structure containing a promoter sequence and crystallized from Mg2+ ions. It reveals sequence-dependent DNA conformations not seen previously, including kinking into the DNA major groove.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available