Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 46, Issue 3, Pages 261-266Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2015.1015568
Keywords
Arctic bacterium; carbohydrate CPAs; cryoprotectant; cryoprotective properties; exopolysaccharide; Pseudoalteromonas elyakovii
Categories
Funding
- project titled Korea-Polar Ocean Development: K-POD [PM14050]
- Ministry of Oceans and Fisheries, Korea
Ask authors/readers for more resources
Twenty-two bacterial strains that secrete exopolysaccharides (EPS) were isolated from marine samples obtained from the Chukchi Sea in the Arctic Ocean; of these, seven strains were found to be capable of producing cryoprotective EPS. The ArcPo 15 strain was isolated based on its ability to secrete large amounts of EPS, and was identified as Pseudoalteromonas elyakovii based on 16S rDNA analysis. The EPS, P-ArcPo 15, was purified by protease treatment and gel filtration chromatography. The purified EPS (P-ArcPo 15) had a molecular mass of 1.7x10(7) Da, and its infrared spectrum showed absorption bands of hydroxyl and carboxyl groups. The principal sugar components of P-ArcPo 15 were determined to be mannose and galacturonic acid, in the ratio of 3.3:1.0. The cryoprotective properties of P-ArcPo 15 were characterized by an Escherichia coli viability test. In the presence of 0.5% (w/v) EPS, the survival percentage of E. coli cells was as high as 94.19 +/- 7.81% over five repeated freeze-thaw cycles. These biochemical characteristics suggest that the EPS P-ArcPo 15 may be useful in the development of cryoprotectants for biotechnological purposes, and we therefore assessed the utility of this novel cryoprotective EPS.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available