Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 46, Issue 8, Pages 780-787Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2015.1135462
Keywords
Aspergillus oryzae; glyphosate degradation endoenzyme; response surface methodology; UV mutagenesis
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Funding
- National Natural Science Foundation of China [31160336]
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This study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. Spore from Aspergillus oryzae A-F02 was subjected to ultraviolet mutagenesis. Single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. Four mutant strains were obtained and named as FUJX 001, FUJX 002, FUJX 003, and FUJX 004, in which FUJX 001 gave the highest total enzyme activity. Starch concentration at 0.56%, GP concentration at 1,370mg/l, initial pH at 6.8, and temperature at 30 degrees C were the optimum conditions for the improved glyphosate degradation endoenzyme production of A. oryzae FUJX 001. Under these conditions, the experimental endoenzyme activity was 784.15U/100ml fermentation liquor. The result (784.15U/100ml fermentation liquor) was approximately 14-fold higher than that of the original strain. The result highlights the potential of glyphosate degradation enzyme to degrade glyphosate.
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