Journal
POLYMER CHEMISTRY
Volume 7, Issue 28, Pages 4693-4702Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6py00814c
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Funding
- NSF Graduate Research Fellowship Program [2012141051]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1352485] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1359321] Funding Source: National Science Foundation
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Recently, we reported a new class of biodegradable, thermoresponsive polyesters (TR-PEs) inspired by polyacrylamides and elastin-like peptides (ELPs). The polyesters exhibit tunable cloud point temperatures (T-cp) and thermoresponsive coacervation in aqueous solution as shown via UV-vis spectroscopy, H-1 NMR, and DLS. However, the T-cp of all TR-PEs remained low (<15 degrees C), and higher thermoresponsivity would be beneficial for many applications. This study examines the synthesis, polymerization, and analysis of a new monomer bearing a more hydrophilic pendant group, bis-2-methoxyethylamine (bMoEtA). The resulting TR-PE, TR-bMoEtAPE, displays a threefold increase in T-cp (ca. 50 degrees C) that is affected by solution (DI water vs. phosphate buffered saline), concentration (1-40 mg mL(-1)) molecular weight (20-130 kDa), and cosolutes (Hofmeister salts and urea). The T-cp and T-g of random TR-bMoEtAPE copolymers can be tuned via comonomer feed. Variable temperature 1H NMR indicated a cooperative coacervation mechanism above T-cp, further reinforced by DLS measurements. As evidenced by UV-vis and SEC analysis, TR-bMoEtAPE underwent rapid degradation over a period of 7 days in DI water and PBS. Finally, cytotoxicity studies suggested that TR-bMoEtAPE is non-cytotoxic even at high concentrations (ca. 1000 mu g mL(-1)). The increased T-cp and tunability suggests TR-bMoEtAPE as a potential candidate for future functionalized TR-PE therapeutic-delivery systems.
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