Journal
PLOS ONE
Volume 11, Issue 1, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0146191
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Funding
- Grants-in-Aid for Scientific Research [26460970, 16K09282] Funding Source: KAKEN
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Background CD5(+) B cells are a type of regulatory immune cells, though the involvement of this B cell subset in intestinal inflammation and immune regulation is not fully understood. Methods We examined the distribution of CD5(+) B cells in various mouse organs. Expression levels of CD11b, IgM, and toll-like receptor (TLR)-4 and -9 in B cells were evaluated. In vitro, TLR-stimulated IL-10 production by colonic lamina propria (LP) CD5(+) and CD5(-) B cells was measured. In vivo, mice with acute or chronic dextran sulfate sodium (DSS)-induced colonic injury were examined, and the frequency of colonic LP CD5(+) B cells in those was assessed by flow cytometry. Results The expression level of TLR9 was higher in colonic LP CD5(+) B cells as compared to CD5(-) B cells. Colonic LP CD5(+) B cells produced greater amounts of IL-10 following stimulation with TLR ligands, especially TLR9, as compared with the LP CD5(-) B cells. Acute intestinal inflammation transiently decreased the frequency of colonic LP CD5(+) B cells, while chronic inflammation induced a persistent decrease in colonic LP CD5(+) B cells and led to a CD5(-) B cell-dominant condition. Conclusion A persistent altered mucosal B cell population caused by chronic gut inflammation may be involved in the pathogenesis of inflammatory bowel diseases.
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