Journal
PLASMA PROCESSES AND POLYMERS
Volume 14, Issue 7, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppap.201600123
Keywords
amine coatings; cell adhesion; cyclopropylamine; ESCA/XPS; pulsed discharges
Funding
- Ministry of Education, Youth and Sports of the Czech Republic [LQ1601, LD15150]
- People Programme (Marie Curie Action) of the Seventh Framework Programme of EU [291782]
- South Moravian Centre for International Mobility, SoMoPro II [3SGA5652]
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Plasma polymers with NHx groups were deposited from cyclopropylamine in low pressure radio frequency (RF) discharges. The amount of NHx decreased with increasing average power P-av, whereas the layer dissolution in water decreased and high P-av led to a slight film swelling. The C2C12 cells demonstrated very high adhesion to the layers regardless of the deposition conditions. Although the surface chemistry of amine-rich layers should enhance the cell proliferation, the WST-1 assay, and immunocytochemistry revealed lower cell proliferation and viability on the layers exhibiting above 18% of relative thickness loss in water. Promising results were obtained on the layers with better water stability and bearing 7-10% of NHx.
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