Journal
PLANT SCIENCE
Volume 246, Issue -, Pages 62-69Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2016.01.011
Keywords
Horizontal gene transfer; RNA plant virus; Cucumber mosaic virus; Soybean; Transposon
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Funding
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- GenoSoja consortium (CNPq) [5527/2007-8]
- GenoProt (CNPq) [559636/2009-1]
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- CNPq [307868/2011-7]
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Horizontal gene transfer (HGT) is known to be a major force in genome evolution. The acquisition of genes from viruses by eukaryotic genomes is a well-studied example of HGT, including rare cases of non-retroviral RNA virus integration. The present study describes the integration of cucumber mosaic virus RNA-1 into soybean genome. After an initial metatranscriptomic analysis of small RNAs derived from soybean, the de novo assembly resulted a 3029-nt contig homologous to RNA-1. The integration of this sequence in the soybean genome was confirmed by DNA deep sequencing. The locus where the integration occurred harbors the full RNA-1 sequence followed by the partial sequence of an endogenous mRNA and another sequence of RNA-1 as an inverted repeat and allowing the formation of a hairpin structure. This region recombined into a retrotransposon located inside an exon of a soybean gene. The nucleotide similarity of the integrated sequence compared to other Cucumber mosaic virus sequences indicates that the integration event occurred recently. We described a rare event of non-retroviral RNA virus integration in soybean that leads to the production of a double-stranded RNA in a similar fashion to virus resistance RNAi plants. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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