Journal
PLANT PHYSIOLOGY
Volume 171, Issue 3, Pages 1704-1719Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.16.00359
Keywords
-
Categories
Funding
- Ghent University Multidisciplinary Research Partnership Sustainable BioEconomy [01MRB510W]
- Interuniversity Attraction Poles Program [IUAP P7/29]
- Research Foundation-Flanders [G0D7914N]
- VIB International PhD Program
- Omics@VIB Marie Curie COFUND
- FWO
- Marie Curie Intra-European Fellowships for Career Development [PIEF-GA-2009-235827]
- Deutsche Forschungsgemeinschaft through the Forscher-gruppe [FOR 1186]
- Academy of Finland Centre of Excellence Program [2014-19]
- IWT (project Phoenix)
- IWT (project Stressnet)
Ask authors/readers for more resources
The genes coding for the core metabolic enzymes of the photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered in genetic screens aimed to isolate mutants that are unviable under ambient air. As an exception, glycolate oxidase (GOX) mutants with a photorespiratory phenotype have not been described yet in C3 species. Using Arabidopsis (Arabidopsis thaliana) mutants lacking the peroxisomal CATALASE2 (cat2-2) that display stunted growth and cell death lesions under ambient air, we isolated a second-site loss-of-function mutation in GLYCOLATE OXIDASE1 (GOX1) that attenuated the photorespiratory phenotype of cat2-2. Interestingly, knocking out the nearly identical GOX2 in the cat2-2 background did not affect the photorespiratory phenotype, indicating that GOX1 and GOX2 play distinct metabolic roles. We further investigated their individual functions in single gox1-1 and gox2-1 mutants and revealed that their phenotypes can be modulated by environmental conditions that increase the metabolic flux through the photorespiratory pathway. High light negatively affected the photosynthetic performance and growth of both gox1-1 and gox2-1 mutants, but the negative consequences of severe photorespiration were more pronounced in the absence of GOX1, which was accompanied with lesser ability to process glycolate. Taken together, our results point toward divergent functions of the two photorespiratory GOX isoforms in Arabidopsis and contribute to a better understanding of the photorespiratory pathway.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available