Journal
PLANT PHYSIOLOGY
Volume 172, Issue 2, Pages 1003-1018Publisher
OXFORD UNIV PRESS INC
DOI: 10.1104/pp.16.00754
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Funding
- Research Grants Council of Hong Kong [CUHK466011, 465112, 466613, CUHK2/CRF/11G, C4011-14R, HKUST10/CRF/12R, AoE/M-05/12, HKUST12/CRF/13G]
- NSFC/RGC [N_CUHK406/12]
- NSFC [31470294, 31570001]
- Croucher-CAS Joint Lab
- Shenzhen Peacock Project [KQTD201101]
- Natural Science Foundation of Guangdong Province [2016A030313401]
- [HKUST VPRGO12SC02]
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Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway that mediates cell wall formation during cell expansion and cytokinesis. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that Golgi-derived secretory vesicles (GDSVs) labeled by NtPPME1-GFP are distinct from those organelles belonging to the conventional post-Golgi exocytosis pathway. In addition, pharmaceutical treatments, superresolution imaging, and dynamic studies suggest that NtPPME1 follows a polar exocytic process from Golgi-GDSV-PM/cell plate (CP), which is distinct from the conventional Golgi-TGN-PM/ CP secretion pathway. Further studies show that ROP1 regulates this specific polar exocytic pathway. Taken together, we have demonstrated an alternative TGN-independent Golgi-to-PM polar exocytic route, which mediates secretion of NtPPME1 for cell wall formation during cell expansion and cytokinesis and is ROP1-dependent.
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