Journal
PLANT PHYSIOLOGY
Volume 172, Issue 2, Pages 1249-1258Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.16.00415
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Funding
- Alabama Agricultural Experiment Station HATCH grant [370222-310010-2055, 370225-310006-2055]
- Auburn University Cellular and Molecular Biosciences Research Fellowship
- FWO [12N2415N]
- Interuniversity Attraction Poles Programme [IUAP P7/29 MARS]
- Belgian Science Policy Office
- Ghent University (Multidisciplinary Research Partnership Biotechnology for a Sustainable Economy) [01MRB510W]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1557796] Funding Source: National Science Foundation
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Cytokinin is a phytohormone that is well known for its roles in numerous plant growth and developmental processes, yet it has also been linked to abiotic stress response in a less defined manner. Arabidopsis (Arabidopsis thaliana) Cytokinin Response Factor 6 (CRF6) is a cytokinin-responsive AP2/ERF-family transcription factor that, through the cytokinin signaling pathway, plays a key role in the inhibition of dark-induced senescence. CRF6 expression is also induced by oxidative stress, and here we show a novel function for CRF6 in relation to oxidative stress and identify downstream transcriptional targets of CRF6 that are repressed in response to oxidative stress. Analysis of transcriptomic changes in wild-type and crf6 mutant plants treated with H2O2 identified CRF6-dependent differentially expressed transcripts, many of which were repressed rather than induced. Moreover, many repressed genes also show decreased expression in 35S:CRF6 overexpressing plants. Together, these findings suggest that CRF6 functions largely as a transcriptional repressor. Interestingly, among the H2O2 repressed CRF6-dependent transcripts was a set of five genes associated with cytokinin processes: (signaling) ARR6, ARR9, ARR11, (biosynthesis) LOG7, and (transport) ABCG14. We have examined mutants of these cytokinin-associated target genes to reveal novel connections to oxidative stress. Further examination of CRF6-DNA interactions indicated that CRF6 may regulate its targets both directly and indirectly. Together, this shows that CRF6 functions during oxidative stress as a negative regulator to control this cytokinin-associated module of CRF6-dependent genes and establishes a novel connection between cytokinin and oxidative stress response.
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