Journal
PLANT PATHOLOGY JOURNAL
Volume 32, Issue 1, Pages 8-15Publisher
KOREAN SOC PLANT PATHOLOGY
DOI: 10.5423/PPJ.OA.08.2015.0157
Keywords
ABC transporter gene; biopesticide; blasticidin S; fungicide resistance; Lysimachia foenum-graecum Herba; Magnaporthe oryzae; plant extract; rice blast disease
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Funding
- Business for Cooperative R&D between Industry, Academy, and Research Institute - Korea Small and Medium Business Administration [00045438]
- Korea Evaluation Institute of Industrial Technology (KEIT) [00045438] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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To identify a novel biopesticide controlling rice blast disease caused by Magnaporthe oryzae, 700 plant extracts were evaluated for their inhibitory effects on mycelial growth of M. oryzae. The L. foenum-graecunz Herba extract showed the lowest inhibition concentration (IC50) of 39.28 mu g/ml, which is lower than the IC50 of blasticidin S (63.06 mu g/ml), a conventional fungicide for rice blast disease. When treatments were combined, the IC50 of blasticidin S was dramatically reduced to 10.67 mu g/ml. Since ABC transporter genes are involved in fungicide resistance of many organisms, we performed RT-PCR to investigate the transcriptional changes of 40 ABC transporter family genes of M. oryzae treated with the plant extract, blasticidin S, and tetrandrine, a recognized ABC transporter inhibitor. Four ABC transporter genes were prominently activated by blasticidin S treatment, but were suppressed by combinational treatment of blasticidin S with the plant extract, or with tetrandrine that didn't show cellular toxicity by itself in this study. Mycelial death was detected via confocal microscopy at 24 h after plant extract treatment. Finally, subsequent rice field study revealed that the plant extract had high control efficacy of 63.3% and should be considered a biopesticide for rice blast disease. These results showed that extract of L. foenunz graecum Herba suppresses M. oryzae ABC transporter genes inducing mycelial death and therefore may be a potent novel biopesticide.
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