Journal
PLANT PATHOLOGY JOURNAL
Volume 32, Issue 2, Pages 162-167Publisher
KOREAN SOC PLANT PATHOLOGY
DOI: 10.5423/PPJ.NT.10.2015.0224
Keywords
bacterial canker; biovar; kiwifruit; molecular marker; Pseudomonas syringae pv. actinidiae
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Funding
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio Industry Technology Development Program - Ministry of Agriculture, Food, and Rural Affairs (MAFRA) [315019-02-1-SB020]
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Pseudomonas syringae pv. actinidiae, the causal agent of canker in kiwifruit, can be divided into three biovars (biovars 1, 2, and 3). Strains belonging to biovar 1 produce phaseolotoxin and were isolated in Japan and Italy before 2008. Strains of biovar 2 produce coronatine instead of phaseolotoxin and have been isolated only in Korea. Strains belonging to biovar 3 produce neither phaseolotoxin nor coronatine and are responsible for the global outbreak of bacterial canker of kiwifruit in recent years. The biovar 3-specific primer set was developed in a previous work. In this study, two sets of PCR primers specific to strains of biovars 1 and 2, respectively, were developed based on random amplified polymorphic DNA analyses. Primers PsaJ-F and PsaJ-R produced a 481-bp region with genomic DNA of biovar 1 strains, whereas primers PsaK-F and PsaK-R amplified a 413-bp region present only in the genome of biovar 2 strains.
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