Journal
PLANT PATHOLOGY
Volume 66, Issue 5, Pages 835-841Publisher
WILEY
DOI: 10.1111/ppa.12628
Keywords
bacterial wilt; leaf-clip inoculation; Ralstonia solanacearum; tomato seedling infection
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Funding
- Department of Biotechnology, Government of India [BT/301/NE/TBP/2012]
- Indo-French project grant from Indo-French Centre for the Promotion of Advanced Research, India (CEFI-PRA) [4800-B1]
- University Grants Commission, Government of India
- Department of Biotechnology, Government of India
- departmental projects UGC-SAP (DSR II)
- DST-FIST (Phase I)
- DBT-Strengthening NE
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Ralstonia solanacearum is a phytopathogenic bacterium that colonizes the xylem vessels of host plants leading to a lethal wilt disease. Although several studies have investigated the virulence of R. solanacearum on adult host plants, infection studies of this pathogen on the seedling stages of hosts are less common. In a preliminary observation, inoculation of R. solanacearum F1C1 on 6- to 7-day-old tomato seedlings by a simple leaf-clip strategy resulted in a lethal pathogenic condition in seedlings that eventually killed these seedlings within a week post-inoculation. This prompted testing of the effect of this inoculation technique in seedlings from different cultivars of tomato and similar results were obtained. Colonization and spread of the bacteria throughout the infected seedlings was demonstrated using gus-tagged R. solanacearum F1C1. The same method of inoculating tomato seedlings was used with R. solanacearum GMI1000 and independent mutants of R. solanacearum GMI1000, deficient in the virulence genes hrpB, hrpG, phcA and gspD. Wildtype R. solanacearum GMI1000 was found to be virulent on tomato seedlings, whereas the mutants were found to be non-virulent. This leaf-clip technique, for inoculation of tomato seedlings, has the potential to be a valuable approach, saving time, space, labour and costs.
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