Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 35, Issue 2, Pages 224-236Publisher
SPRINGER
DOI: 10.1007/s11105-016-1014-y
Keywords
UDP-glucosyltransferase gene; Hordeum vulgare L; Fusarium head blight (FHB); Deoxynivalenol (DON); In vitro antifungal activity; Virus-induced gene silencing (VIGS)
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Funding
- Important National Science and Technology Specific Projects of Transgenic Research [2014ZX0800202B-002]
- Jiangsu Natural Science Foundation [BK2011636, BK20140700]
- National University Doctoral Fund of China [20100097120036]
- Jiangsu Science and Technology Support Program [BE2013439]
- Jiangsu Agricultural Science and Technology Innovation Fund [CX151001]
- Program of Introducing Talents of Discipline to Universities [B08025]
- Anhui Provincial Natural Science Foundation [1408085QC64]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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UDP-glucosyltransferases (UGTs) contribute to Fusarium head blight (FHB) resistance of wheat and barley by glycosylating the deoxynivalenol (DON), which is produced by Fusarium fungus. In this study, seven alleles of barley HvUGT14077 (GenBank No.GU170356.1) were cloned using RT-PCR. Among them, HvUGT-10W1, which was isolated from a FHB resistant barley variety 10W1, was significantly up-regulated in young spikes after F. graminearum (F.g) inoculation. HvUGT-10W1::GFP was subcellularly located in the plasma membrane and cytoplasm of the wheat protoplasts. In vitro antifungal activity assay showed that the HvUGT-10W1 protein exerted obvious inhibition against the growth of F.g. The silencing of the HvUGT-10W1 by virus-induced gene silencing (VIGS) resulted in compromised FHB resistance of 10W1, which was shown by the increased infected colonies on the leaves. These indicated that the barley HvUGT-10W1 may also contribute to F.g resistance in barley and provided a potential candidate gene to develop transgenic barley with enhanced FHB resistance.
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