Journal
PLANT JOURNAL
Volume 89, Issue 1, Pages 112-127Publisher
WILEY-BLACKWELL
DOI: 10.1111/tpj.13326
Keywords
protein phosphatase 2A; glucosinolate; activated methyl cycle; Arabidopsis thaliana
Categories
Funding
- Academy of Finland [271832, 273870, 251397, 256073, 283254]
- University of Turku Doctoral Program in Molecular Life Sciences
- DFG Heisenberg Fellowship [GL346/5]
- TUM Junior Fellow Fund
- FPU Fellowship [AP2010-5857]
- Natural Sciences and Engineering Research Council of Canada
- Academy of Finland (AKA) [283254, 251397, 256073, 251397, 283254, 256073] Funding Source: Academy of Finland (AKA)
Ask authors/readers for more resources
Glucosinolates (GSL) of cruciferous plants comprise a major group of structurally diverse secondary compounds which act as deterrents against aphids and microbial pathogens and have large commercial and ecological impacts. While the transcriptional regulation governing the biosynthesis and modification of GSL is now relatively well understood, post-translational regulatory components that specifically determine the structural variation of indole glucosinolates have not been reported. We show that the cytoplasmic protein phosphatase 2A regulatory subunit B'gamma (PP2A-B'gamma) physically interacts with indole glucosinolate methyltransferases and controls the methoxylation of indole glucosinolates and the formation of 4-meth-oxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves. By taking advantage of proteomic approaches and metabolic analysis we further demonstrate that PP2A-B'gamma is required to control the abundance of oligomeric protein complexes functionally linked with the activated methyl cycle and the trans-methylation capacity of leaf cells. These findings highlight the key regulatory role of PP2A-B'gamma in methionine metabolism and provide a previously unrecognized perspective for metabolic engineering of glucosinolate metabolism in cruciferous plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available