Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 126, Issue 2, Pages 319-331Publisher
SPRINGER
DOI: 10.1007/s11240-016-1000-y
Keywords
Flow cytometry; Gentiana kurroo; Gentiana tibetica; Polyploidy; Protoclonal variation; Somatic embryogenesis
Funding
- Polish Ministry of Science and Higher Education [3P04C 037 23]
Ask authors/readers for more resources
The morphogenic potential of five Gentiana species of medicinal and ornamental value, i.e.G. cruciata, G. kurroo, G. lutea, G. septemfida, and G. tibetica, was compared using agarose-bead leaf mesophyll protoplast culture. Modified Murashige and Skoog medium containing 2.0 mg l(-1) 1-naphthaleneacetic acid and 0.1 mg l(-1) thidiazuron ensured the highest cell division frequency during both protoplast and protoplast-derived cell culture or callus formation. Indirect plant regeneration mainly via the somatic embryogenesis pathway was achieved for G. kurroo and G. tibetica with the greatest efficiency occurring with MS medium supplemented with 1.0 mg l(-1) kinetin, 0.5 mg l(-1) gibberellic acid, and 80 mg l(-1) adenine sulfate. A considerable percentage of autopolyploid and aneuploid cells was detected in callus lines obtained from protoplasts using flow cytometry. The presence of polyploid cells in calli resulted in the regeneration of 85 % polyploid plants of G. kurroo and 14 % polyploids of G. tibetica. Chromosome counting and stomatal characteristic confirmed the ploidy variation among regenerants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available