Journal
PLANT CELL AND ENVIRONMENT
Volume 39, Issue 5, Pages 1140-1160Publisher
WILEY
DOI: 10.1111/pce.12726
Keywords
antioxidative enzymes; ascorbate; DNA damage; glutathione; hydrogen peroxide; lipid peroxides; NAD(P); reactive oxygen species (ROS); redox proteomics
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Funding
- BBSRC (UK) [BB/M009130/1]
- French Agence Nationale de la Recherche [ANR-12-BSV6-0011]
- Biotechnology and Biological Sciences Research Council [BB/M009130/1] Funding Source: researchfish
- BBSRC [BB/M009130/1] Funding Source: UKRI
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Oxidative stress and reactive oxygen species (ROS) are common to many fundamental responses of plants. Enormous and ever-growing interest has focused on this research area, leading to an extensive literature that documents the tremendous progress made in recent years. As in other areas of plant biology, advances have been greatly facilitated by developments in genomics-dependent technologies and the application of interdisciplinary techniques that generate information at multiple levels. At the same time, advances in understanding ROS are fundamentally reliant on the use of biochemical and cell biology techniques that are specific to the study of oxidative stress. It is therefore timely to revisit these approaches with the aim of providing a guide to convenient methods and assisting interested researchers in avoiding potential pitfalls. Our critical overview of currently popular methodologies includes a detailed discussion of approaches used to generate oxidative stress, measurements of ROS themselves, determination of major antioxidant metabolites, assays of antioxidative enzymes and marker transcripts for oxidative stress. We consider the applicability of metabolomics, proteomics and transcriptomics approaches and discuss markers such as damage to DNA and RNA. Our discussion of current methodologies is firmly anchored to future technological developments within this popular research field. Summary Statement Oxidative stress and related redox processes have become integrated into many parts of plant biology research. Here, we provide a critical methodological evaluation of some of the approaches that are used to monitor, gauge and dissect oxidative stress and related redox signalling in plants. Our Forward Look review discusses current obstacles to progress and foreseeable technological developments that are likely to promote ever faster advances within this intensely studied area.
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