Journal
PLANT CELL
Volume 28, Issue 2, Pages 454-465Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.15.00570
Keywords
-
Funding
- BBSRC
- Biotechnology and Biological Sciences Research Council [BB/I002243/1, BB/E012582/1, BB/L014130/1] Funding Source: researchfish
- BBSRC [BB/L014130/1, BB/E012582/1, BB/I002243/1] Funding Source: UKRI
Ask authors/readers for more resources
C-4 photosynthesis is a complex phenotype that allows more efficient carbon capture than the ancestral C-3 pathway. In leaves of C-4 species, hundreds of transcripts increase in abundance compared with C-3 relatives and become restricted to mesophyll (M) or bundle sheath (BS) cells. However, no mechanism has been reported that regulates the compartmentation of multiple enzymes in M or BS cells. We examined mechanisms regulating CARBONIC ANHYDRASE4 (CA4) in C-4 Gynandropsis gynandra. Increased abundance is directed by both the promoter region and introns of the G. gynandra gene. A nine-nucleotide motif located in the 5' untranslated region (UTR) is required for preferential accumulation of GUS in M cells. This element is present and functional in three additional 5' UTRs and six 3' UTRs where it determines accumulation of two isoforms of CA and pyruvate, orthophosphate dikinase in M cells. Although the GgCA4 5' UTR is sufficient to direct GUS accumulation in M cells, transcripts encoding GUS are abundant in both M and BS. Mutating the GgCA4 5' UTR abolishes enrichment of protein in M cells without affecting transcript abundance. The work identifies a mechanism that directs cell-preferential accumulation of multiple enzymes required for C-4 photosynthesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available