Journal
PLANT BIOLOGY
Volume 18, Issue 3, Pages 471-483Publisher
WILEY-BLACKWELL
DOI: 10.1111/plb.12427
Keywords
Biochemical growth elicitors; bio-formulation; cold and drought stress; gene expression; induced resistance; rice
Categories
Funding
- Zhejiang Provincial Natural Science Foundation of China [R13C140001]
- Zhejiang Provincial Project [2014C32010]
- National Natural Science Foundation of China [31571971, 31371904]
- Fundamental Research Funds for the Central Universities
- Agricultural Ministry of China [nyhyzx 201303015, 201003029, 201003066]
- Key Subject Construction Program of Zhejiang for Modern Agricultural Biotechnology and Crop Disease Control [2010DS700124-KF1101, 2010DS700124-KF1203, 2010DS700124-KF1309, 2010DS700124-KF1410]
Ask authors/readers for more resources
In the present study, a consortium of two rhizobacteria Bacillus amyloliquefaciens Bk7 and Brevibacillus laterosporus B4, termed BB', biochemical elicitors salicylic acid and -aminobutyric acid (SB) and their mixture (BBSB) were investigated for cold and drought stress tolerance in rice plants. After withholding water for 16days, rice plants treated with BBSB showed 100% survival, improved seedling height (35.4cm), shoot number (6.12), and showed minimum symptoms of chlorosis (19%), wilting (4%), necrosis (6%) and rolling of leaves. Similarly, BB inoculation enhanced plant growth and reduced overall symptoms in rice seedlings subjected to 0 +/- 5 degrees C for 24h. Our results imply several mechanisms underlying BB- and BBSB-elicited stress tolerance. In contrast to the control, both treatments significantly decreased leaf monodehydroascorbate (MDA) content and electrolyte leakage, and increased leaf proline and cholorophyll content. Moreover, activities of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) increased 3.0- and 3.6-fold, respectively. Moreover, expression of OsMYB3R-2, OsDIL, OsDREB1A and OsCDPK13 genes was significantly up-regulated, suggesting that these genes play important roles in abiotic stress tolerance of rice. In addition, bacterial strains Bk7 and B4 were able to produce high amounts of IAA and siderophores, and colonise the plant roots, while only strain Bk7 exhibited the capability to form biofilms and solubilise inorganic phosphate. This study indicates that the BB and BBSB bio-formulations can be used to confer induced systematic tolerance and improve the health of rice plants subject to chilling and drought stress.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available