Journal
PLANT AND CELL PHYSIOLOGY
Volume 58, Issue 1, Pages 106-119Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcw183
Keywords
Cellulose; Cell wall; Image analysis; Mathematical model; Microtubules; Pavement cell
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science [25711017, 26520207, 26891006, 24114007, 25291056, 15K14536]
- Grants-in-Aid for Scientific Research [25711017, 16H04802, 26891006, 15K14536, 24114007, 26520207, 16K18562] Funding Source: KAKEN
Ask authors/readers for more resources
Pavement cells in cotyledons and true leaves exhibit a jigsaw puzzle-like morphology in most dicotyledonous plants. Among the molecular mechanisms mediating cell morphogenesis, two antagonistic Rho-like GTPases regulate local cell outgrowth via cytoskeletal rearrangements. Analyses of several cell wall-related mutants suggest the importance of cell wall mechanics in the formation of interdigitated patterns. However, how these factors are integrated is unknown. In this study, we observed that the application of exogenous cellulase to hydroponically grown Arabidopsis thaliana cotyledons switched the interdigitation of pavement cells to the production of smoothly elongated cells. The cellulase-induced inhibition of cell interdigitation was not observed in a RIC1 knockout mutant. This gene encodes a Rho-like GTPase-interacting protein important for localized cell growth suppression via microtubule bundling on concave cell interfaces. Additionally, to characterize pavement cell morphologies, we developed a mathematical model that considers the balance between cell and cell wall growth, restricted global cell growth orientation, and regulation of local cell outgrowth mediated by a Rho-like GTPase-cytoskeleton system. Our computational simulations fully support our experimental observations, and suggest that interdigitated patterns form because of mechanical buckling in the absence of Rho-like GTPase-dependent regulation of local cell outgrowth. Our model clarifies the cell wall mechanics influencing pavement cell morphogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available