Journal
PIGMENT CELL & MELANOMA RESEARCH
Volume 29, Issue 5, Pages 559-571Publisher
WILEY-BLACKWELL
DOI: 10.1111/pcmr.12506
Keywords
melanocytes; actindynamics; migration; vesicle traffic; endocytic trafficking
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Funding
- Cancer Research UK [A15673, A15672]
- Biological Services Unit [A17196]
- Cancer Research UK [15673, 15672] Funding Source: researchfish
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The five-subunit WASH complex generates actin networks that participate in endocytic trafficking, migration and invasion in various cell types. Loss of one of the two subunits WASH or strumpellin in mice is lethal, but little is known about their role in mammals in vivo. We explored the role of strumpellin, which has previously been linked to hereditary spastic paraplegia, in the mouse melanocytic lineage. Strumpellin knockout in melanocytes revealed abnormal endocytic vesicle morphology but no impairment of migration in vitro or in vivo and no change in coat colour. Unexpectedly, WASH and filamentous actin could still localize to vesicles in the absence of strumpellin, although the shape and size of vesicles was altered. Blue native PAGE revealed the presence of two distinct WASH complexes, even in strumpellin knockout cells, revealing that the WASH complex can assemble and localize to endocytic compartments in cells in the absence of strumpellin.
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