Journal
PHYTOCHEMICAL ANALYSIS
Volume 28, Issue 3, Pages 195-201Publisher
WILEY
DOI: 10.1002/pca.2660
Keywords
Ocimum basilicum; reverse iontophoresis; ion chromatography; mass spectrometry; plant metabolite identification
Funding
- Spanish Government (MINECO) [BFU2013-44095-P]
- Spanish Government (FEDER funds, European Union)
- Regional Ministry of Education and Science of Castilla-La Mancha
- Regional Ministry of Education and Science of Castilla-La Mancha (European Social Fund, Programa Operativo FSE)
- BBSRC [BB/R000344/1] Funding Source: UKRI
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Introduction - The identification and characterisation of cellular metabolites has now become an important strategy to obtain insight into functional plant biology. However, the extraction of metabolites for identification and analysis is challenging and, at the present time, usually requires destruction of the plant. Objective - To detect different plant metabolites in living plants with no pre-treatment using the combination of iontophoresis and ion-chromatography with mass spectrometry detection. Methodology - In this work, the simple and non-destructive method of reverse iontophoresis has been used to extract in situ multiple plant metabolites from intact Ocimum basilicum leaves. Subsequently, the analysis of these metabolites has been performed with ion chromatography coupled directly to high resolution mass spectrometric detection (IC-MS). Results - The application of reverse iontophoresis to living plant samples has avoided the need for complex pre-treatments. With this approach, no less than 24 compounds, including organic acids and sugars as well as adenosine triphosphate (ATP) were successfully detected. Conclusion - The research demonstrates that it is feasible to monitor, therefore, a number of important plant metabolites using a simple, relatively fast and non-destructive approach. Copyright (C) 2016 John Wiley & Sons, Ltd.
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