4.6 Article

Refolding effects of partially immiscible ammonium-based ionic liquids on the urea-induced unfolded lysozyme structure

Journal

PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Volume 18, Issue 18, Pages 12419-12422

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cp01022a

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Funding

  1. Department of Biotechnology, New Delhi, India [BT-PR5287/BRB/10/1068/2012]

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The activity of lysozyme over a Micrococcus lysodeikticus cell suspension increased to 13% of the initial value in the presence of 1% v/v ammonium-based ionic liquids after deactivation with 4.0 M urea. This increase in activity reflects the refolding ability of the ionic liquids against the denaturation effects of urea on lysozyme.

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