Journal
CHEMOSPHERE
Volume 126, Issue -, Pages 32-39Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2015.01.055
Keywords
BDE-47; HEK293 cells; Cytotoxicity; Cell apoptosis; ROS; Metabolomics
Categories
Funding
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020405]
- Key Research Program of the Chinese Academy of Sciences [KZZD-EW-14]
- National Natural Science Foundation of China [21107136]
- International Foundation for Science [F/5230-1]
Ask authors/readers for more resources
Polybrominated diphenyl ethers (PBDEs) are widely used as brominated flame-retardants in a variety of industrial products. Among these PBDEs, 2,2',4,4'-tetra-bromodiphenyl ether (BDE-47) is one of the most predominant congeners inducing multiple toxicities, including hepatotoxicity, neurotoxicity, cytotoxicity, genotoxicity, carcinogenecity and immunotoxicity in human body. In this study, the cytotoxicity of BDE-47 in human embryonic kidney cells (HEK293) was investigated by a set of bioassays, including cell proliferation, apoptosis, oxidative stress and metabolic responses as well as gene expressions related to apoptosis. Results showed that BDE-47 induced an inverted U-shaped curve of cell proliferation in HEK293 cells from 10(-6) to 10(-4) M. Cell apoptosis and ROS overproduction were detected at 10(-5) M of BDE-47 (p < 0.05). In addition, the expressions of Bcl-2 family-encoding genes (Bad, Hrk and Bcl-2) increased significantly in 10(-4) M group (p <0.05). Metabolic responses indicated that BDE-47 mainly caused disturbance in energy metabolism marked by differentially altered ethanol, glutathione, creatine, aspartate, UDP-glucose and NAD(+). The increased lactatefalanine ratios indicated the higher reductive state induced by BDE-47 in all exposures confirmed by the overproduction of ROS. (C) 2015 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available