Journal
CHEMOSPHERE
Volume 121, Issue -, Pages 26-32Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2014.10.053
Keywords
Extracellular polymeric substances (EPS); Flocs; Granular sludge; Three-dimensional spectroscopy (EEM); Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
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Funding
- National Natural Science Foundation of China [51078327, 51478416]
- Natural Science Foundation of Zhejiang Province [Y13E080003]
- National Key Technologies Research and Development Program of China [2013BAC16B04]
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Extracellular polymeric substances (EPS) plays an important role in the formation of bioaggregates such as flocs, biofilm and granular sludge. However, the role of their specific components in sludge flocculation and granulation is still unclear. Three sludge samples including the flocs, aerobic and anaerobic granular sludge were investigated in this study and the specific components in different EPS structures of loosely bound-EPS (LB-EPS) and tightly bound-EPS (TB-EPS) were analyzed. Results showed that the protein (PN) contents in LB-EPS and TB-EPS of the aerobic and anaerobic granular sludge were 33.6 +/- 9.7 and 96.8 +/- 11.9, 27.1 +/- 2.8 and 61.6 +/- 4.2 mg g(-1) VSS, respectively, which were both higher than the flocs of 8.5 +/- 1.5 and 43.1 +/- 2.7 mg g(-1) VSS. But the polysaccharide (PS) contents in the three sludges were all about 30 mg g(-1) VSS. The analysis of sludge surface charge indicated that they had a linear correlation with the PN content, which implied that PN significantly contributed to the formation of granular sludge. The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that the molecular weight of PN in flocs was mainly distributed in 14.3-66.2 kDa, while it was 20.1-97.4 kDa in the granular sludge, which indicated that the proteins with high molecular weight favors the sludge granulation. According to the three-dimensional fluorescence (EEM) results, the aromatic protein-like and tryptophan protein-like substances were more abundant in the granular sludge than that in flocs, suggesting they are the key components in the structural stability of granular sludge. (C) 2014 Elsevier Ltd. All rights reserved.
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