Journal
PHARMACEUTICAL RESEARCH
Volume 33, Issue 10, Pages 2400-2410Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11095-016-1963-8
Keywords
atherosclerosis; nanoparticle; natriuretic peptide clearance receptor; polymer synthesis; positron emission tomography
Funding
- National Heart, Lung and Blood Institute of the National Institutes of Health [HHSN268201000046C]
- MRSEC Program of the National Science Foundation [DMR1121053]
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To assess the physicochemical properties, pharmacokinetic profiles, and in vivo positron emission tomography (PET) imaging of natriuretic peptide clearance receptors (NPRC) expressed on atherosclerotic plaque of a series of targeted, polymeric nanoparticles. To control their structure, non-targeted and targeted polymeric (comb) nanoparticles, conjugated with various amounts of c-atrial natriuretic peptide (CANF, 0, 5, 10 and 25%), were synthesized by controlled and modular chemistry. In vivo pharmacokinetic evaluation of these nanoparticles was performed in wildtype (WT) C57BL/6 mice after Cu-64 radiolabeling. PET imaging was performed on an apolipoprotein E-deficient (ApoE(-/-)) mouse atherosclerosis model to assess the NPRC targeting efficiency. For comparison, an in vivo blood metabolism study was carried out in WT mice. All three Cu-64-CANF-comb nanoparticles showed improved biodistribution profiles, including significantly reduced accumulation in both liver and spleen, compared to the non-targeted Cu-64-comb. Of the three nanoparticles, the 25% Cu-64-CANF-comb demonstrated the best NPRC targeting specificity and sensitivity in ApoE(-/-) mice. Metabolism studies showed that the radiolabeled CANF-comb was stable in blood up to 9 days. Histopathological analyses confirmed the up-regulation of NPRC along the progression of atherosclerosis. The 25% Cu-64-CANF-comb demonstrated its potential as a PET imaging agent to detect atherosclerosis progression and status.
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