Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 132, Issue -, Pages 72-80Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2015.11.002
Keywords
Panonychus citri; Glutathione S-transferases; Abamectin resistance; RNA interference; Metabolism assay
Categories
Funding
- Special Fund for Agro-scientific Research in the Public Interest [201203038]
- National Natural Science Foundation [31171851]
- earmarked fund for the Modern Agro-industry (Citrus) Technology Research System of China
- Fundamental Research Funds for the Central Universities of China [XDJK2013A017]
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The citrus red mite, Panonychus citri (McGregor), a major citrus pest distributed worldwide, has been found to be resistant to various insecticides and acaricides used in China. However, the molecular mechanisms associated with the abamectin resistance in this species have not yet been reported. In this study, results showed over expression of a novel glutathione S-transferases (GSTs) gene (PcGSTm5) in abamectin-resistant P. citri. Quantitative real-time PCR analysis showed that the transcripts of PcGSTm5 were also significantly up-regulated after exposure to abamectin and the maximum mRNA expression level at nymphal stage. The recombinant protein of PcGSTm5-pET-28a produced by Escherichia coli showed a pronounced activity toward the conjugates of 1-chloro-2,4 dinitrobenzene (CDNB) and glutathione (GSH). The kinetics of CDNB and GSH and its optimal pH and thermal stability were also determined. Reverse genetic study through a new method of leaf-mediated dsRNA feeding further support a link between the expression of PcGSTm5 and abamectin resistance. However, no direct evidence was found in metabolism or inhibition assays to confirm the hypothesis that PcGSTm5 can metabolize abamectin. Finally, it is here speculated that PcGSTm5 may play a role in abamectin detoxification through other pathway such as the antioxidant protection. (C) 2015 Elsevier Inc. All rights reserved.
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