Journal
PEST MANAGEMENT SCIENCE
Volume 73, Issue 1, Pages 53-63Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.4365
Keywords
systemic RNAi; small hive beetle (Aethina tumida); Laccase 2; V-ATPase subunit A; European honey bee (Apis mellifera)
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Funding
- UK Department for Environment, Food and Rural Affairs (Defra)
- BBSRC [BB/M027147/1] Funding Source: UKRI
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BACKGROUNDAethina tumida is a serious pest of the European honey bee (Apis mellifera) in North America and Australia. Here we investigate whether Laccase 2, the phenoloxidase gene essential for cuticle sclerotisation and pigmentation in many insects, and vacuolar-ATPase V-type subunit A, vital for the generation of proton gradients used to drive a range of transport processes, could be potential targets for RNAi-mediated control of A. tumida. RESULTSInjection of V-ATPase subunit A (5 ng) and Laccase 2 (12.5 ng) dsRNAs resulted in 100% larval mortality, and qPCR confirmed significant decreases and enhanced suppression of transcript levels over time. Oral delivery of V-ATPase subunit A dsRNA in solutions resulted in 50% mortality; however, gene suppression could not be verified. We suggest that the inconsistent RNAi effect was a consequence of dsRNA degradation within the gut owing to the presence of extracellular nucleases. Target specificity was confirmed by a lack of effect on survival or gene expression in honey bees injected with A. tumida dsRNAs. CONCLUSIONThis is the first study to show evidence for systemic RNAi in A. tumida in response to injected dsRNA, but further research is required to develop methods to induce RNAi effects via ingestion. (c) 2016 Crown copyright. Pest Management Science (c) 2016 Society of Chemical Industry
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