Journal
CHEMMEDCHEM
Volume 10, Issue 9, Pages 1559-1563Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cmdc.201500241
Keywords
hyperpolarization; ligand binding; NMR spectroscopy; saturation transfer difference
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Funding
- National Science Foundation [CHE-0846402, CHE-0840464]
- Welch Foundation [A-1658]
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Nuclear magnetic resonance (NMR) spectroscopy is a valuable technique for ligand screening, because it exhibits high specificity toward chemical structure and interactions. Dissolution dynamic nuclear polarization (DNP) is a recent advance in NMR methodology that enables the creation of non-equilibrium spin states, which can dramatically increase NMR sensitivity. Here, the transfer of such spin polarization from hyperpolarized ligand to protein is observed. Mixing hyperpolarized benzamidine with the serine protease trypsin, a fingerprint of enhanced protein signals is observed, which shows a different intensity profile than the equilibrium NMR spectrum of the protein, but coincides closely to the frequency profile of a saturation transfer difference (STD) NMR experiment. The DNP experiment benefits from hyperpolarization and enables observation of all frequencies in a single, rapid experiment. Based on these merits, it is an interesting alternative to the widely used STD experiment for identification of protein-ligand interactions.
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