Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 21, Issue 11, Pages 4315-4320Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201405658
Keywords
aggregation; fluorescent probes; imaging agents; microviscosity; sensors
Categories
Funding
- National Basic Research Program of China (973 Program) [2013CB834701]
- Research Grants Council of Hong Kong [16301614, 604913, 604711, 6602212, N_HKUST604/14, N_HKUST620/11]
- University Grants Committee of Hong Kong [AoE/P-03/08]
- Guangdong Innovative Research Team Program [201101C0105067115]
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Intracellular viscosity is a crucial parameter that indicates the functioning of cells. In this work, we demonstrate the utility of TPE-Cy, a cell-permeable dye with aggregation-induced emission (AIE) property, in mapping the viscosity inside live cells. Owing to the AIE characteristics, both the fluorescence intensity and lifetime of this dye are increased along with an increase in viscosity. Fluorescence lifetime imaging of live cells stained with TPE-Cy reveals that the life-time in lipid droplets is much shorter than that from the general cytoplasmic region. The loose packing of the lipids in a lipid droplet results in low viscosity and thus shorter lifetime of TPE-Cy in this region. It demonstrates that the AIE dye could provide good resolution in intracellular viscosity sensing. This is also the first work in which AIE molecules are applied in fluorescence lifetime imaging and intracellular viscosity sensing.
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