Adipose-Derived Stromal Cells from Lipomas: Isolation, Characterisation and Review of the Literature

Objective: The aim of this study was to characterize adipose-derived stromal cells (ADSCs) from patients diagnosed with multiple symmetric lipomatosis (MSL) in order to obtain potentially new insights into the pathophysiology, pathogenesis and treatment of this disease. Methods: Cells from the stromal vascular fraction were analysed by the colony-forming efficiency assay and flow cytometry using standard markers. Moreover, the power of adipogenic plasticity was evaluated. Finally, a literature review was performed from 1982 to 2015 using the US National Institutes of Health's PubMed database. Results: Three European-descent patients diagnosed with either MSL type I or II could be identified for analysis. The resulting mean colony-forming efficiency assay was 14.3 +/- 5%. Flow-cytometric analysis of the ADSCs revealed high levels of CD34 (70 +/- 9%), CD45 (37 +/- 13%) and CD73 (55.8 +/- 14%), whereas low levels of CD31 (16.8 +/- 14%) and CD105 (5.8 +/- 0.7%) were detected. Furthermore, ADSCs showed a strong adipogenic potential, which is in line with the literature review. The stem cell pool in lipoma shows several alterations in biological activities, such as proliferation, apoptosis and stemness. Conclusions: ADSCs from lipoma may be interesting in the application of regenerative medicine. We discuss possible molecular treatment options to regulate their activities at the source of the MSL. (C) 2016 S. Karger AG, Basel