Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 21, Issue 48, Pages 17158-17171Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201501531
Keywords
heterocycles; luciferins; luminescence; natural products; photoproteins
Categories
Funding
- Agence Nationale de la Recherche (ANR) [ANR-11-CRNT-0004]
- Institut Carnot Pasteur-Maladies Infectieuses
- Institut Carnot Curie-Cancer
- Institut Carnot Voir et Entendre
- Institut Carnot Institut du Cerveau et de la moelle Epiniere
- Institut Carnot Consortium pour l'Acceleration de l'Innovation et de son Transfert dans le domaine du Lymphome (CALYM)
- Agence Nationale de la Recherche (ANR) [ANR-11-CRNT-0004] Funding Source: Agence Nationale de la Recherche (ANR)
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In the last few decades, bioluminescent systems based on the expression of a luciferase and the addition of a luciferin to monitor the emission of light have become very important tools for biological investigations. A growing proportion of these systems use coelenterazine or analogues of imidazo[1,2-a]pyrazine luciferins along with photoproteins or luciferases from sea creatures such as Aequorea, Renilla, Gaussia or Oplophorus. Central to the success of these tools are the synthetic pathways developed not only to prepare the naturally occurring luciferins, but also to design altered compounds that exhibit improved bioluminescence. Current work is indeed focused on the design of systems exhibiting extended luminescence (glow systems) or redshifted wavelengths, as well as constructions better adapted to conditions in cells or in vivo. This review describes the synthetic pathways used to prepare imidazo[1,2-a]pyrazine luciferins along with the research efforts aimed at preparing analogues even better suited to the design of assays.
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