Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 21, Issue 33, Pages 11664-11670Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201501489
Keywords
biosynthesis; chlorophyll; phyllobilin; structure elucidation; tetrapyrrole
Categories
Funding
- Science Foundations of Austria (FWF) [L-472, I-563]
- Science Foundations of Switzerland (SNF) [31003A_132603, 31003A_149389]
- Austrian Science Fund (FWF) [I 563] Funding Source: researchfish
- Swiss National Science Foundation (SNF) [31003A_132603] Funding Source: Swiss National Science Foundation (SNF)
Ask authors/readers for more resources
1-Formyl-19-oxobilin-type tetrapyrroles are characteristic, abundant products of chlorophyll breakdown in senescent leaves. However, in some leaves, 1,19-dioxobilin-type chlorophyll catabolites (DCCs) lacking the formyl group accumulate instead. A P450 enzyme was identified in in vitro studies that removed the formyl group of a primary fluorescent chlorophyll catabolite (pFCC) and generated fluorescent DCCs. These DCCs are precursors of isomeric nonfluorescent DCCs (NDCCs). Here, we report a structural investigation of the NDCCs in senescent leaves of wild-type Arabidopsis thaliana. Four new NDCCs were characterized, two of which carried a stereoselectively added hydroxymethyl group. Such formal DCC hydroxymethylations were previously found in DCCs in leaves of a mutant of A.thaliana. They are now indicated to be a feature of chlorophyll breakdown in A.thaliana, associated with the specific in vivo deformylation of pFCC en route to NDCCs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available