4.6 Article

Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

Journal

ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 14, Issue 17, Pages 4023-4026

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ob00533k

Keywords

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Funding

  1. FONDECYT [1110809, 1140240]
  2. CSIC-USACH [2010CL0013]
  3. NSERC
  4. Canada Research Chairs program
  5. Canada Foundation for Innovation
  6. UBA
  7. CONICET
  8. DFAIT (Canada) fellowship from ELAP (Emerging Leaders in the Americas Program)

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A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.

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