Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 14, Issue 17, Pages 4023-4026Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ob00533k
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Funding
- FONDECYT [1110809, 1140240]
- CSIC-USACH [2010CL0013]
- NSERC
- Canada Research Chairs program
- Canada Foundation for Innovation
- UBA
- CONICET
- DFAIT (Canada) fellowship from ELAP (Emerging Leaders in the Americas Program)
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A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered.
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