Journal
OPTICS LETTERS
Volume 41, Issue 7, Pages 1427-1430Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.41.001427
Keywords
-
Categories
Funding
- Fundamental Research Funds for the Central Universities [JUSRP115A14]
- Natural Science Foundation of Jiangsu Province of China [BK2012548]
- Scientific Research Foundation of Shanghai University of Electric Power [K2014018]
Ask authors/readers for more resources
Since quantitative phase distribution reflects both cellular shapes and conditions from another view, compared to traditional intensity observation, different quantitative phase microscopic methods are proposed for cellular detections. However, the transport of intensity equation-based approach not only presents phase, but also intensity, which attracts much attention. While classical transport of intensity equation needs multi-focal images which often cannot realize simultaneous phase measurement, in this Letter, to break through the limitation, a real-time quantitative phase imaging method using transport of intensity equation is proposed. Two identical CCD cameras are set at the binocular tubes to capture the same field of view but at different focal planes. With a double-frame algorithm assuming that the on-focal image is the average of over-and under-focal information, the proposed method is capable of calculating quantitative phase distributions of samples accurately and simultaneously indicating its potentialities in cellular real-time monitoring. (C) 2016 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available