Journal
OPTICS LETTERS
Volume 42, Issue 1, Pages 13-16Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.42.000013
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Funding
- National Natural Science Foundation of China (NSFC) [51473078]
- National Program for Support of Top-Notch Young Professionals, Scientific and Technological Innovation Teams of Colleges and Universities in Jiangsu Province [TJ215006]
- Natural Science Foundation of Jiangsu Province of China [BK20130038]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [YX03001]
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A reducing intracellular environment is necessary for living cells. Here a redox-sensitive phosphorescent probe Ir-NO has been developed for evaluating the redox environment in living cells. Upon addition of reducing molecules, such as glutathione and ascorbic acid, the phosphorescent intensity of the probe is turned on, and the emission lifetime is elongated evidently. Furthermore, this probe has been used for optical imaging of the intracellular reducing environment by utilizing confocal laser scanning microscopy and phosphorescence lifetime imaging microscopy. (C) 2016 Optical Society of America
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