4.6 Article

Single-shot and phase-shifting digital holographic microscopy using a 2-D grating

Journal

OPTICS EXPRESS
Volume 24, Issue 9, Pages 9480-9488

Publisher

OPTICAL SOC AMER
DOI: 10.1364/OE.24.009480

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Funding

  1. Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - the Ministry of Health & Welfare, Republic of Korea [HI14C3477, HI13C1501]
  2. Korea University [K1505441]

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We demonstrate digital holographic microscopy that, while being based on phase-shifting interferometry, is capable of single-shot measurements. A two-dimensional (2-D) diffraction grating placed in a Fourier plane of a standard in-line holographic phase microscope generates multiple copies of a sample image on a camera sensor. The identical image copies are spatially separated with different overall phase shifts according to the diffraction orders. The overall phase shifts are adjusted by controlling the lateral position of the grating. These phase shifts are then set to be multiples of pi/2. Interferograms composed of four image copies combined with a parallel reference beam are acquired in a single shot. The interferograms are processed through a phase-shifting algorithm to produce a single complex image. By taking advantage of the higher sampling capacity of the in-line holography, we can increase the imaging information density by a factor of 3 without compromising the imaging acquisition speed. (C) 2016 Optical Society of America

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