Journal
OPTICAL AND QUANTUM ELECTRONICS
Volume 48, Issue 6, Pages -Publisher
SPRINGER
DOI: 10.1007/s11082-016-0610-2
Keywords
Raman spectroscopy; PLS regression; Blood proteins; Albumin; Immunoglobulin
Funding
- Ministry of Education and Science of the Russian Federation
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This work is dedicated to the measurement of plasma proteins concentration in liquid solutions using Raman spectroscopy setup. The obtained Raman spectra showed significant variation of intensities of certain spectral bands 940, 1005, 1330, 1450 and 1650 cm(-1) for different protein fractions and concentrations. Partial least squares regression analysis was used for determination of correlation coefficients. It has been shown that the proposed method represents structure and biochemical composition of albumin and immunoglobulins A and G, and that albumin concentration may be measured with an accuracy of 6-8 g/L.
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