Journal
ONCOLOGY REPORTS
Volume 37, Issue 1, Pages 631-639Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2016.5237
Keywords
microRNA-302a; insulin-like growth factor-1 receptor; 5-fluorouracil; Akt; human colon cancer cell
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Funding
- National Natural Science Foundation of China [81172358, 31100969]
- Fundamental Research Funds of Xi'an Jiaotong University [xjj2016076]
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New therapeutic strategies are needed for colorectal cancer (CRC) treatment. MicroRNAs are involved in cancer-pertinent cellular processes, including chemoresistance. As miR-302a is an embryonic stem cell-specific microRNA, studies on miR-302a have focused on its role in human stem cells. Studies analyzing miR-302 function in cancer are limited. In this study, we used two human colon cancer cell lines, HCT116 and HT29, and evaluated the influence of miR-302a on 5-fluorouracil (5-FU)-induced cell death and viability inhibition. With bioinformatics tools, we hypothesized that insulin-like growth factor-1 receptor (IGF-1R) is a novel target of miR-302a, which we confirmed using a luciferase reporter assay and immunoblotting. Then, we designed siRNA against IGF-1R and found that si-IGF-1R resembled the effect of miR-302a on 5-FU treatment. Both miR-302a and si-IGF-1R inhibited Akt signaling. In conclusion, miR-302a targeted IGF-1R and enhanced 5-FU-induced cell death and viability inhibition in human colon cancer cells. Targeting miR-302a may offer new therapeutic interventions in CRC.
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