Journal
CHEMISTRY LETTERS
Volume 44, Issue 10, Pages 1437-1439Publisher
CHEMICAL SOC JAPAN
DOI: 10.1246/cl.150544
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Funding
- National Natural Science Foundation of China [20972170, 21275150]
- Funds for Distinguished Young Scientists of Gansu [1210RJDA013]
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7-nitro-2,1,3-benzoxadiazole (NBD)-based fluorescent probe for the detection of cysteine (Cys) over homocysteine (Hey) and amino acids under neutral physiological conditions based on thiol-induced aromatic nucleophilic substitution reaction was synthesized by the reaction of 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) with p-thiocresol. The proposed sensing mechanism was proved by mass spectrometry of the reaction mixtures. This probe was successfully applied to the fluorescence imaging of Cys in HeLa cells.
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