Journal
NUCLEIC ACIDS RESEARCH
Volume 45, Issue D1, Pages D56-D60Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkw913
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Funding
- Swiss National Science Foundation [31003A_162735]
- SyBIT project of SystemsX.ch
- Swiss Federal Institute of Technology in Lausanne (EPFL)
- Swiss National Science Foundation (SNF) [31003A_162735] Funding Source: Swiss National Science Foundation (SNF)
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GETPrime (http://bbcftools.epfl.ch/getprime) is a database with a web frontend providing geneand transcript-specific, pre-computed qPCR primer pairs. The primers have been optimized for genome-wide specificity and for allowing the selective amplification of one or several splice variants of most known genes. To ease selection, primers have also been ranked according to defined criteria such as genome-wide specificity (with BLAST), amplicon size, and isoform coverage. Here, we report a major upgrade (2.0) of the database: eight new species (yeast, chicken, macaque, chimpanzee, rat, platypus, pufferfish, and Anolis carolinensis) now complement the five already included in the previous version (human, mouse, zebrafish, fly, and worm). Furthermore, the genomic reference has been updated to Ensembl v81 (while keeping earlier versions for backward compatibility) as a result of re-designing the back-end database and automating the import of relevant sections of the Ensembl database in species-independent fashion. This also allowed us to map known polymorphisms to the primers (on average three per primer for human), with the aim of reducing experimental error when targeting specific strains or individuals. Another consequence is that the inclusion of future Ensembl releases and other species has now become a relatively straightforward task.
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