Journal
NUCLEIC ACIDS RESEARCH
Volume 44, Issue 6, Pages 2528-2537Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkw137
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Funding
- Home Institute
- European Union (ITN NICHE)
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Premature ribosome drop-off is one of the major errors in translation of mRNA by ribosomes. However, repeated analyses of Ribo-seq data failed to quantify its strength in E. coli. Relying on a novel highly sensitive data analysis method we show that a significant rate of ribosome drop-off is measurable and can be quantified also when cells are cultured under non-stressing conditions. Moreover, we find that the drop-off rate is highly variable, depending on multiple factors. In particular, under environmental stress such as amino acid starvation or ethanol intoxication, the drop-off rate markedly increases.
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