Journal
NUCLEIC ACIDS RESEARCH
Volume 45, Issue 5, Pages 2600-2614Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkw1275
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Funding
- Italian Association of Cancer Research AIRC Grant [IG15868]
- AIRC StartUP [12710]
- University of Zurich
- Swiss National Science Foundation Grant [152621]
- Swiss National Science Foundation [156280]
- ETH Research Commission [ETH-43 14-1]
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Ribonucleotides (rNs) incorporated in the genome by DNA polymerases (Pols) are removed by RNase H2. Cytidine and guanosine preferentially accumulate over the other rNs. Here we show that human Pol eta can incorporate cytidine monophosphate (rCMP) opposite guanine, 8-oxo-7,8-dihydroguanine, 8-methyl-2'- deoxyguanosine and a cisplatin intrastrand guanine crosslink (cis-PtGG), while it cannot bypass a 3-methylcytidine or an abasic site with rNs as substrates. Pol eta is also capable of synthesizing polyribonucleotide chains, and its activity is enhanced by its auxiliary factor DNA Pol delta interacting protein 2 (PolDIP2). Human RNase H2 removes cytidine and guanosine less efficiently than the other rNs and incorporation of rCMP opposite DNA lesions further reduces the efficiency of RNase H2. Experiments with XP-V cell extracts indicate Pol eta as the major basis of rCMP incorporation opposite cis-PtGG. These results suggest that translesion synthesis by Pol eta can contribute to the accumulation of rCMP in the genome, particularly opposite modified guanines.
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