Journal
CHEMISTRY AND PHYSICS OF LIPIDS
Volume 191, Issue -, Pages 115-122Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2015.07.010
Keywords
Lipid; Lipid-protein interaction; Fatty acid; NMR; Metabolism; Bioenergetics
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Funding
- NIH [GM 58688]
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Previous studies have shown that palmitate (PA) can interact with myoglobin (Mb). The present study has investigated the interaction of the more soluble unsaturated fatty acid, oleic acid (OA). Indeed, H-1 NMR measurements of the Mb signal during OA titration also show signal changes consistent with specific and non-specific binding. At OA:Mb ratio <4:1, OA perturbs selectively the 8-heme methyl signal, consistent with a local and specific fatty acid-protein interaction. As OA:Mb ratio increases from 4:1 to 40:1, all hyperfine shifted MbCN signals decrease, consistent with a non-selective, global perturbation of the protein. The pH titration analysis indicates that the observed OA methylene signal in the presence of Mb reflects a non-specific interaction and does not originate from a shift in the lamella micelle equilibrium. Given the OA interaction with Mb, a fatty acid flux model suggests that Mb can play a fatty acid transport role under certain physiological conditions. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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