Guillain-Barre Syndrome Associated with Zika Virus Infection in Colombia

BACKGROUND Zika virus (ZIKV) infection has been linked to the Guillain-Barre syndrome. From November 2015 through March 2016, clusters of cases of the Guillain-Barre syndrome were observed during the outbreak of ZIKV infection in Colombia. We characterized the clinical features of cases of Guillain-Barre syndrome in the context of this ZIKV infection outbreak and investigated their relationship with ZIKV infection. METHODS A total of 68 patients with the Guillain-Barre syndrome at six Colombian hospitals were evaluated clinically, and virologic studies were completed for 42 of the patients. We performed reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assays for ZIKV in blood, cerebrospinal fluid, and urine, as well as antiflavivirus antibody assays. RESULTS A total of 66 patients (97%) had symptoms compatible with ZIKV infection before the onset of the Guillain-Barre syndrome. The median period between the onset of symptoms of ZIKV infection and symptoms of the Guillain-Barre syndrome was 7 days (inter-quartile range, 3 to 10). Among the 68 patients with the Guillain-Barre syndrome, 50% were found to have bilateral facial paralysis on examination. Among 46 patients in whom nerve-conduction studies and electromyography were performed, the results in 36 patients (78%) were consistent with the acute inflammatory demyelinating polyneuropathy subtype of the Guillain-Barre syndrome. Among the 42 patients who had samples tested for ZIKV by RT-PCR, the results were positive in 17 patients (40%). Most of the positive RT-PCR results were in urine samples (in 16 of the 17 patients with positive RT-PCR results), although 3 samples of cerebrospinal fluid were also positive. In 18 of 42 patients (43%) with the Guillain-Barre syndrome who underwent laboratory testing, the presence of ZIKV infection was supported by clinical and immunologic findings. In 20 of these 42 patients (48%), the Guillain-Barre syndrome had a parainfectious onset. All patients tested were negative for dengue virus infection as assessed by RT-PCR. CONCLUSIONS The evidence of ZIKV infection documented by RT-PCR among patients with the Guillain-Barre syndrome during the outbreak of ZIKV infection in Colombia lends support to the role of the infection in the development of the Guillain-Barre syndrome. (Funded by the Bart McLean Fund for Neuroimmunology Research and others.)