Journal
NEW BIOTECHNOLOGY
Volume 33, Issue 4, Pages 460-466Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.nbt.2016.02.005
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Exopolysaccharides (EPS) are polymers excreted by some microorganisms with interesting properties and used in many industrial applications. A new Pseudoalteromonas sp. strain, MD12-642, was isolated from marine sediments and cultivated in bioreactor in saline culture medium containing glucose as carbon source. Its ability to produce EPS under saline conditions was demonstrated reaching an EPS production of 4.4 g/L within 17 hours of cultivation, corresponding to a volumetric productivity of 0.25 g/L h, the highest value so far obtained for Pseudoalteromonas sp. strains. The compositional analysis of the EPS revealed the presence of galacturonic acid (41-42 mol%), glucuronic acid (25-26 mol%), rhamnose (16-22 mol%) and glucosamine (12-16 mol%) sugar residues. The polymer presents a high molecular weight (above 1000 kDa). These results encourage the biotechnological exploitation of strain MD12-642 for the production of valuable EPS with unique composition, using saline by-products/wastes as feedstocks.
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